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To visualise GLP-1Rs in human pericytes, pericytes isolated from cardiac surgery waste tissue (cell isolation methods and characterisation as described previously 66 ) were cultured to semi-confluency, fixed with 4% PFA for 20 min and labelled with the anti-human PDGFR antibody (Santa Cruz, sc-374573, 1:50) and Fluorescent Exendin-4 (Fluorescein-Trp25-Exendin-4, FLEX